It is very difficult to identify 100 invasive species from around the world that really are "worse" than any others. Species and their interactions with ecosystems are very complex. Some species may have invaded only a restricted region, but have a high probability of expanding and causing further great damage (e.g. see Boiga irregularis: the brown tree snake). Other species may already be globally widespread, and causing cumulative but less visible damage. Many biological families or genera contain large numbers of invasive species, often with similar impacts.

Species were selected for the list according to two criteria: their serious impact on biological diversity and/or human activities, and their illustration of important issues surrounding biological invasion. To ensure the inclusion of a wide variety of examples, only one species from each genus was selected. Absence from the list does not imply that a species poses a lesser threat.

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